Contributions of flavonoids from citri reticulatae pericarpium to gastric hormones, CD3~+ and TFF3 mRNA expression in rats with spleen deficiency intervened by Liujunzi Decoction / 中国中药杂志

The present study established the spectrum-effect relationship model of flavonoids in Citri Reticulatae Pericarpium(CRP) from 15 batches of Liujunzi Decoction and statistically analyzed the correlation between chemical peaks and efficacy to identify the main effective components. HPLC fingerprints of flavonoids in CRP from 15 batches of Liujunzi Decoction were established. HPLC analysis was carried out on the Venusil XBP C_(18)(L) column(4.6 mm×250 mm, 5 μm) at 30 ℃ with acetonitrile-water(containing 0.1% formic acid) as mobile phase for gradient elution, a flow rate of 1.0 mL·min~(-1), and detection wavelength of 300 nm to obtain chemical fingerprints. Additionally, the effects of flavonoids from CRP in 15 batches of Liujunzi Decoction on the content of GAS, MTL, and VIP, TFF3 mRNA expression, and percentage of CD3~+ T-cells of model rats with spleen deficiency were determined. The spectrum-effect relationship model was established by gray correlation analysis. The results showed that the main characteristic peaks with great contribution to the regulation of gastrointestinal tract were peak 16(vicenin-2), peak 63(sinensetin), peak 64(isosinensetin), peak 65(nobiletin), peak 67(3,5,6,7,8,3',4'-heptemthoxyflavone), peak 68(tangeretin), and peak 69(5-desmethylnobiletin). Therefore, there was a linear correlation between flavonoids from CRP in Liujunzi Decoction and the efficacy, and the medicinal effect was achieved by multi-component action. This study is expected to provide a new idea for exploring the material basis of the effect, i.e., regulating qi prior to replenishing qi, of CRP in Liujunzi Decoction.

Liujunzi decoction ameliorats cisplatin-induced anorexia via adjusting metabolic disorders in rats / 中国药理学与毒理学杂志

OBJECTIVE Cisplatin is a formidable chemotherapy agent widely applying in antineoplastic treatments, but its side effects often limit the clinical usage. Metabolic disorders are one of the side effects induced by cisplatin, which closely relate to the onset of chemotherapy-induced anorexia (CIA) in cancer patients but lacks effective controls. Liujunzi decoction (LJZD) is a traditional Chinese formula that has a promising effect in treating CIA. However, whether LJZD ameliorates CIA through adjusting cisplatin-induced metabolic disorders remain unknow. The present study evalu?ated the mechanism of cisplatin-induced metabolic disorders, and the effect of LJZD in ameliorating these disturbances. METHODS 42 male Sprague-Dawley (SD) rats (180-220 g) were randomly divided into 3 groups:normal control group (distilled water+saline), model group (distilled water+cisplatin), LJZD group (4.8 g·kg-1 Liujunzi decoction ingredients+cisplatin). Intragastrical administered each drug twice a day (7:00-19:00) since day 0 for 4 d, animals were intraperito?neal injected with cisplatin 6 mg·kg-11 h after administration while normal control groups were injected with same volume of saline. On day 3, each group was anesthetized with pentobarbital sodium 45 mg · kg-1 (ip), and blood samples were collected from aorta abdominalis. Then the samples were analyzed using an LC-ESI-MS/MS system. Significantly regu?lated metabolites between groups were determined by VIP≥1 and absolute Log2FC (fold change)≥1. Identified metabo?lites were mapped to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database using Metaboanalyst 5.0 (https://www.metaboanalyst.ca/). RESULTS A total of 133, 77 and 32 differential metabolites were filtrated in control vs model, control vs LJZD and model vs LJZD groups respectively. Comparing to control, the levels of hexadecanoic acid (Log2FC=6.3153), linoleic acid (Log2FC=5.3478), and 8, 11-icosadienoic acid (Log2FC=5.2342) significantly increased, and the levels of N-acetyl-L-tyrosine (Log2FC = -2.6283), cinnamic acid (Log2FC = -2.3381), N-acetylphenylalanine (Log2FC = -2.2501) significantly decreased in model group. The KEGG pathway enrichments of these metabolites indi?cated that, cisplatin-induced metabolic disorders by disturbing metabolism pathways such as linoleic acid metabolism, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism, which suggested that the onset of CIA was partly associated with the metabolic disorders of linoleic acid, unsaturated fatty acids, and phenylalanine. Compared to control, treatment of LJZD significantly increased the levels of 4-hydroxytryptamine (Log2FC =12.0186), hexadecanoic acid (Log2FC = 5.7412), linoleic acid (Log2FC = 5.1877) and significantly decreased the levels of N-acetylmethionine (Log2FC=-1.7317), 2-aminoethanesulfinic acid (Log2FC=-1.6578), N-acetyl-L-tyrosine (Log2FC=-1.5355). And com?paring to the model group, 4-hydroxytryptamine (Log2FC = 12.0186), 7, 12-diketocholic acid (Log2FC = 2.0998), N-acetylneuraminic acid (Log2FC = 2.0560) markedly increased, and 3-hydroxy-3-methylpentane-1 (Log2FC = -1.9202), 5-dioic acid (Log2FC = -1.7166), N-isovaleroylglycine, hexanoyl glycine (Log2FC = -1.4958) markedly decreased in LJZD group. It was worth noting that, there were 23 differential metabolites filtrated both in control vs model and model vs LJZD groups, which were the key metabolites of LJZD in treating CIA. Among these 23 common metabolites, there were 16 metabolites excluding the control vs LJZD group, that was, LJZD had no effect in normal rats while being able to ameliorated cisplatin-induced metabolic disorders by regulating these 16 metabolites. Cisplatin-induced downregula?tion of 11 metabolites such as hydrocinnamic acid, (±)12(13)epoxy-9Z-octadecenoic acid, cinnamic acid were upregulated after LJZD treatment, and cisplatin-induced upregulation of imidazoleacetic acid, 2'-deoxycytidine-5'-monophosphate and other 5 metabolites were downregulated by LJZD. The KEGG pathway analysis indicated that the linoleic acid metabolism, histidine metabolism, and pyrimidine metabolism were the most enriched metabolic pathway. Thus, cisplatin-induced metabolic disturbances mainly by disturbing linoleic acid metabolism, histidine metabolism, and pyrimidine metabolism, and LJZD interacted with these metabolic pathways to reduce metabolic disorders and thus ameliorated CIA. CONCLUSION Cisplatin-induced anorexia was closely related to the metabolic disorders of linoleic acid metabo?lism, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism. The mechanism of LJZD in ameliorating CIA was in concerned with the metabolic adjustments, relating to the regulation of linoleic acid metabolism, histidine metabolism, and pyrimidine metabolism.

Systematic review and Meta-analysis on efficacy and safety of Liujunzi Decoction combined with Western medicine for stable chronic obstructive pulmonary disease / 中国中药杂志

To systematically review the efficacy and safety of Liujunzi Decoction combined with Western medicine in the treatment of stable chronic obstructive pulmonary disease(COPD). Three English databases and four Chinese databases were systematically searched from the database establishment to April 1, 2020. We screened randomized controlled trial(RCT) according to the pre-determined inclusion and exclusion criteria, then extracted data. Methodological quality of included studies was assessed with Cochrane bias risk evaluation tool. Data were analyzed by using RevMan 5.3. A total of 401 articles were retrieved and finally 17 RCTs were included in this study, involving 1 447 patients, and the overall quality of the included studies was not high. Meta-analysis showed that, in reducing traditional Chinese medicine symptom score, Liujunzi Decoction combined with conventional Western medicine or Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation was superior to conventional Western medicine or Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation alone. In reducing the grade of modified medical research council(mMRC), Liujunzi Decoction combined with Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation was superior to Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation alone. In reducing COPD assessment test(CAT) score, Liujunzi Decoction combined with conventional Western medicine was superior to conventional Western medicine alone. In delaying the decline of forced expiratory volume in one second(FEV_1) or % in the expected value, Liujunzi Decoction combined with conventional Western medicine or Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation was superior to conventional Western medicine or Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation alone. In delaying the decline of ratio of FEV_1 to forced vital capacity(FEV_1/FVC), Liujunzi Decoction combined with conventional Western medicine was superior to conventional Western medicine alone, but there was no statistical difference between Liujunzi Decoction combined with Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation and Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation alone. In reducing acute exacerbation rate, there was no statistical difference between Liujunzi Decoction combined with Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation and Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation alone. On the other outcome measures of Liujunzi Decoction combined with other Western medicine, Meta-analysis could not be conducted and conclusions due to the inclusion of only one study. In terms of the occurrence of adverse reactions, some studies did not mention, so the safety of Liujunzi Decoction combined with Wes-tern medicine could not be determined in this paper. Due to the limitations of the quality and quantity of inclu-ded studies, the efficacy of Liujunzi Decoction combined with Western medicine for COPD still needs more high-quality studies for confirmation, and its safety needs to be further verified.

Clinical Study on Chaishao Liujunzi Decoction Combined with Chemotherapy in Treating Advanced Pancreatic Cancer / 中国中医药信息杂志

Objective To observe and analyze the clinical efficacy of Chaishao Liujunzi Decoction combined with chemotherapy in the treatment of advanced pancreatic cancer with liver depression and spleen deficiency. Methods Totally 60 cases of advanced pancreatic cancer patients were divided into treatment group and control group according to random number table method, with 30 cases in each group. Both groups were given nutritional support, to maintain electrolyte balance, three levels of painkiller and anti-infective and other basic treatment. Patients in the treatment group were treated with capecitabine monotherapy, 1250 mg/m2 per time, twice a day, 30 min after dinner, orally, for two weeks and one week off for a chemotherapy cycle, a total of two cycles of chemotherapy. While patients in the treatment group were additionally treated with Chaishao Liujunzi Decoction, 1 dosage per day for morning and evening, for 6 weeks. Followed up for 4 weeks. Efficacy of solid tumor, TCM symptom efficacy and serum gastrointestinal cancer antigen (CA19-9) in two groups were compared; KPS scores before and after treatment were observed; chemotherapy toxicity was monitored. Results Totally 59 patients completed the observation and the control group lost 1 case. After treatment, the tumor stability rate of the control group and the treatment group were 76.7％ (23/30) and 65.5％ (19/29) respectively, without statistical significance (P>0.05). The total effective rate of symptom efficacy was 90％ (27/30) in the treatment group and 69％ (20/29) in the control group, with statistical significance (P<0.05). The efficacy of serum19-9 of the treatment group was better than the control group (P<0.05). 2 groups had varying degrees of chemotherapy toxicity (P<0.05). Conclusion Chaishao Liujunzi Decoction combined with chemotherapy in the treatment of advanced pancreatic cancer can improve the main clinical symptoms, decrease serum CA19-9, and reduce the adverse reactions caused by chemotherapy.

Curative effect of Guishao Liujunzi decoction combined with entecavir tablets in the treatment of liver cirrhosis with hepatitis B / 中国基层医药

Objective@#To observe the clinical effect of Guishao Liujunzi decoction combined with entecavir tablets in the treatment of liver cirrhosis with hepatitis B, and to provide reference for the clinical treatment.@*Methods@#From May 2012 to January 2015, 80 patients with hepatitis B-induced liver cirrhosis treated in Dongyang Guangfu Hospital were selected, and they were divided into control group(n=40) and observation group(n=40) according to the different method.The control group was given entecavir tablets, while the observation group was given Guishao Liujunzi decoction on this basis.The TCM symptom scores and levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), gamma glutamyl transferase(GGT), total bilirubin(TBil), prothrombin time(PT), hepatitis B virus deoxyribonucleic acid(HBV-DNA) of the two groups before treatment and 3 months, 6 months and 12 months after treatment were compared.@*Results@#The TCM symptom scores of the control group at 3 months, 6 months and 12 months after treatment were (12.68±1.82)points, (12.07±1.74)points, (11.38±1.63)points, respectively, which were higher than that before treatment[(14.30±1.48)points, t=4.368, 6.174, 8.388, all P<0.05]. The TCM symptom scores of the observation group at 3 months, 6 months and 12 months after treatment were (11.25±1.63)points, (10.40±1.82)points, (9.81±1.47)points, respectively, which were higher than before treatment[(14.59±1.61)points, t=9.137, 10.828, 13.780, all P<0.05]. The TCM symptom scores of the observation group at 3 months, 6 months and 12 months after treatment were significantly lower than those of the control group(t=3.589, 4.220, 4.359, all P<0.05). After treatment, the levels of ALT, AST, GGT and TBil in the observation group were (39.24±28.37)U/L, (45.18±34.35)U/L, (41.63±10.55)U/L, (20.94±9.15)μmol/L, respectively, which were lower than (85.18±27.22)U/L, (109.25±37.09)U/L, (50.71±9.62)U/L, (33.97±10.11)μmol/L in the control group(t=7.184, 7.818, 3.905, 5.898, all P<0.05). The levels of HBV-DNA in the control group at 6 months and 12 months after treatment were (3.18±1.13) copies/mL and (2.25±0.51) copies/mL, respectively, which were significantly lower than (3.65±1.19)copies/mL before treatment(t=1.811, 6.839; all P<0.05). The levels of HBV-DNA in the observation group at 6 months and 12 months after treatment were (2.96±0.97)copies/mL and (2.07±0.81)copies/mL, respectively, which were significantly lower than (3.70±1.13)copies/mL before treatment(t=3.143, 7.415, all P<0.05).@*Conclusion@#Guishao Liujunzi decoction combined with entecavir tablets in the treatment of liver cirrhosis with hepatitis B not only can significantly improve the clinical symptoms of patients, but also can help to strengthen the antiviral effect, improve the liver function of patients, which has important significance to the prognosis.

Ameliorating effect of Xionggui Liujunzi Decoction on coronary heart disease in rats / 中国比较医学杂志

Objective To explore the effect of a Chinese Medicine Xionggui Liujunzi Decoction on the experimental coronary heart disease in rats. Methods Rats were randomly divided into five groups: the blank control group, the model control group, the western medicine control group (simvastatin), the Chinese medicine control group ( compound Danshen dripping pills) and the Xionggui Liujunzi Decoction group. The coronary heart disease was induced by intragastric gavage of fat emulsion (10 mL/kg, q. d. for 12 weeks) and pituitrin (30 U/kg, q. d. for 3 days) was intraperitoneally injected to induce coronary artery spasm. Changes of the ST segment in electrocardiogram (ECG) and the blood lipids were detected, and the levels of the inflammatory factors including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), highly sensitive C-reactive protein (hs-CRP), monocyte chemotactic protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1), the vascular endothelial active substances including ET-1, NO, TXA2 and PGI2, and the indicators of fibrinolytic system function such as PAI-1, t-PA in the plasma were measured. Results Compared with the model control group, each drug treatment showed better effects on the ST segment of the electrocardiogram, blood lipids, levels of the inflammatory factors and vascular endothelial active substances, and the fibrinolytic function, with significant differences (P ＜ 0. 05). In particular, Xionggui Liujunzi Decoction has a significantly better effect than the compound Danshen dripping pills and simvastatin (P＜ 0. 05). Conclusions Xionggui Liujunzi Decoction can improve the ST segment of the electrocardiogram, blood lipids, levels of the vascular endothelial active substances and fibrinolytic function of the rat model of coronary heart disease, and alleviate inflammation responses, showing a significant effect on coronary arteriosclerosis and myocardial ischemia in rats.

Clinical Observation of Predominant Clinical Efficacy of Xiang Sha Liujunzi Decoction for Treatment of Chronic Gastritis / 广州中医药大学学报

Objective To investigate the predominant clinical efficacy of syndrome differentiation treatment with Xiang Sha Liujunzi Decoction in treating chronic gastritis by observing its curative effect on traditional Chinese medicine(TCM) symptoms and gastroscopy of chronic gastritis pationts . Methods Ninety cases of chronic gastritis patients were randomly divided into control group and treatment group,each group having 45 cases. The control group received Omeprazole Tablets combined with Domperidone Tablets orally,4 weeks constituting a course of treatment. For the patients in the control group with Helicobacter pylori(HP)positive,Amoxicillin Capsules and Metronidazole Tablets were added, and the antibiotics were suspended after one week. The treatment group was given syndrome differentiation treatment with Xiang Sha Liujunzi Decoction on the basis of treatment for the control group. After one course of treatment,the scores of TCM symptoms were evaluated and the gastroscopy was re-examined. Results (1)After one course of treatment, the total effective rate for TCM symptoms in the treatment group was 93.3%, and that in the control group was 66.7%, the difference being statistically significant(P < 0.05).(2)The total effective rate for gastroscopy in the treatment group was 86.7%, and that in the control group was 68.9%,the difference being statistically significant(P < 0.05).(3)The results of Mann-Whitney U rank-sum test showed that the effects on TCM symptoms were superior to the effects on gastroscopy in the treatment group, the difference being statistically significant(Z=-1.974, P < 0.05). However, the differences of the two kinds of clinical efficacy in the control group were not statistically significant(Z =-0.662, P > 0.05), indicating that the application of Chinese medicine in the treatment group had obvious effect on relieving TCM symptoms. Conclusion Compared to western medicine alone, syndrome differentiation treatment with Xiang Sha Liujunzi Decoction plus western medicine can enhance the therapeutic effect in treating chronic gastritis. The application of Chinese medicine alone or combined with western medicine exerts predominant efficacy on relieving TCM symptoms of chronic gastritis patients,in particular for the patients mainly with the manifestations of self-perceived discomforts.

Clinical Study of Acupoint Catgut-embedding Therapy for Prevention and Treatment of Bone Marrow Depression in Breast Cancer Patients Induced by FEC Chemotherapy / 广州中医药大学学报

Objective To compare the clinical efficacy of acupoint catgut-embedding therapy plus fundamental treatment versus fundamental treatment alone for the prevention and treatment of bone marrow depression in breast cancer patients induced by FEC(fluorouracil, epirubicin and cyclophosphamide) chemotherapy. Methods A total of 46 post-operation breast cancer patients accepting the first cycle of FEC chemotherapy were randomly divided into treatment group and control group, 23 cases in each group. The control group was given fundamental treatment including oral use of Chinese medicine of modified Xiangsha Liujunzi Decoction for regulating stomach and arresting vomiting, modified Guipi Decoction and Gui Lu Erxian Decoction for nourishing blood and generating blood, and western medicine of Batilol Tablets and Vitamin B4 Tablets. The treatment group was given acupoint catgut-embedding therapy on bilateral Zusanli(ST36) and Shenshu(BL23) plus fundamental treatment. The effect on bone marrow depression in the two groups was observed after treatment. Results(1) On post-chemotherapy day 7 and 8, the count of white blood cells (WBC) and neutrophils (NE) in the treatment group was higher than that in the control group, the difference being significant(P < 0.05 or P < 0.01).(2) The utilization of granulocyte colony-stimulating factor(G-CSF) in the treatment group was less than that in the control group, the difference being significant (P < 0.05). (3) On post-chemotherapy day 10, the effect on improving bone marrow depression in the treatment group was superior to that in the control group, the difference being significant (P<0.01) . Conclusion The acupoint catgut-embedding therapy plus fundamental treatment is more effective and safe for the prevention and treatment of bone marrow depression in breast cancer patients induced by FEC chemotherapy than fundamental treatment alone.

Effect of Modified Liujunzi Decoction on Syndrome of Spleen Deficiency and Phlegm Stagnation in Patients with Type 2 Diabetes Mellitus / 中国中医药信息杂志

Objective To observe the effects of modified Liujunzi Decoction on improving isletβ-cell function of patients with the syndrome of spleen-deficiency and phlegm-stagnation of type2 diabetes mellitus.Methods Totally 72 patients were selected and divided into treatment group and control group randomly, with 36 cases in each group. Both groups were under diet adjustment and appropriate exercise and were given metformin hydrochloricle tablets, 0.5 g each time (1.0 g each time after 2 weeks), twice a day; acarbose, 50 mg each time, three times a day. The treatment group was given modified Liujunzi Decoction additionally, 200 mL each time, three times a day, orally. The course of treatment of the two groups was three months. Blood glucose, C peptide, INS, HbA1c, HOMA-IR, HOMA-β andΔI30/ΔG30 after 30 minutes of glucagon loading was recorded.Results Compared with before treatment, FBG, 30 min PBG, 2h PBG, HbA1c, FINS, fasting C-peptide, and HOMA-IR decreased in both groups, and the 30 min INS, 30 min C peptide, andΔI30/ΔG30 increased in the treatment group, with statistical significance (P<0.05). After treatment, there was statistical significance in FBG, 30 min PBG, 2 h PBG, HbA1c, fasting C-peptide, FINS, 30 min INS, 30 min C peptide, HOMA-IR, andΔI30/ΔG30 between the two groups (P<0.05).Conclusion Modified Liujunzi Decoction combined with Western medicine can better improve isletβ-cell function of patients with the syndrome of spleen-deficiency and phlegm-stagnation of type2 diabetes mellitus than Western medicine merely.

Clinical Observation of Modified Liujunzi Decoction in Adjuvant Chemotherapy for Advanced Non-small Cell Lung Cancer / 中国药房

OBJECTIVE:To observe the therapeutic efficacy and safety of modified Liujunzi decoction in adjuvant chemotherapy for advanced non-small cell lung cancer (NSCLC).METHODS:A total of 118 patients with advanced NSCLC were randomly divided into observation group and control group,with 59 cases in each group.Both groups were given Dexamethasone acetate tablets 15 mg orally 12,6 h before chemotherapy,Diphenhydramine hydrochloride injection 20 mg intramuscularly 30 min before chemotherapy,and also given routine treatment as acid suppression,liver protection,antiemetic,hydration and dieresis.Control group was additionally given Paclitaxel injection 175 mg/m2 intravenously within 3 h,d1,and 30 min later given Cisplatin for injection 25 mg/m2 intreavenously for 2 h,d1-3.Observation group was additionally given modified Liujunzi decoction,decocted to 200 mL,one dose a day,morning and night,on the basis of control group.A treatment course of 2 groups lasted for 21 d,and both received 4 courses.The short-term efficacies of 2 groups were observed.The levels of T lymphocyte subset and tumor marker (CEA,SCC-Ag,CYFRS21-1),the occurrence of toxic reaction were observed before and after chemotherapy.RESULTS:Total response rate of observation group (49.15％) was significantly higher than that of control group(32.20％);the incidence of thrombocytopenia,leucopenia,abnormal liver function,digestive tract reaction and peripheral nerve injury was significantly lower than control group,with statistical significance (P＜ 0.05).After treatment,CD4+ and CD4+/CD8+ of observation group were significantly higher than before chemotherapy,while CD8+ was significantly lower than before chemotherapy;CD4+ and CD4+/CD8+ of control group were significantly lower than before chemotherapy,while CD8+ was significantly higher than before chemotherapy;CD4+ and CD4+/ CD8 + of observation group were significantly higher than those control group,while CD8 + was significantly lower than control group;The levels of tumor markers in 2 groups were significantly lower than before chemotherapy,and the observation group was significantly lower than the control group,with statistical significance (P＜0.05).CONCLUSIONS:Based on routine treatment,modified Liujunzi decoction shows significant therapeutic efficacy for NSCLC,improves immune function and relieve toxic reaction.

Experience of Making Formula and Description on Treatment of Gastric Abscess in Zhang Yuqing’ Medical Records / 浙江中医药大学学报

Objective]The article analyzed and summed up the experience of making formula and description on treatment of gastric abscess in Qing Dynasty Zhang Yuqing’s Medical Records, in order to provide reference in treating contemporary similar diseases.[Methods]Through studying the medical cases of this book,the authors selected out 30 cases of gastric abscess for futher analysis and summing up the Zhang Yuqing’s experience of description.[Result]He usually used the methods to cure gastric abscess, i.e. expelling phlegm by Banxi and Hualou,harmonizing stomach and liver by Zuojin pills, removing obstruction in channels by Xufuhua decoction, tonifying spleen by Liujunzi decoction and Weiling decoction.These cases had accurate effect and distinct treatment characteristics.[Conclusion]Zhang had rich experience in treating gastric abscess, those can broaden insight in treating similar diseases by traditional Chinese medicine,and increase clinical efficacy.

The progress of pharmacological and clinical researches on Xiangsha-Liujunzi decoction / 国际中医中药杂志

To observe the progess of pharmacological and clinical researches on Xiangsha-Liujunzi decoction by literature reviews recently. Pharmacological study found that the Xiangsha-Liujunzi decoction can protect the gastric mucosa, regulate gastrointestinal endocrine function, promote apoptosis of hyperplasia epithelial cell lying the gastric mucosa, and promote antidepressant effect. Thus, Xiangsha-Liujunzi decoction could be used for gastrointestinal diseases and tumor postoperative gastrointestinal dysfunction caused by radiation and chemotherapy.

Effects of Xiangsha Liujunzi Decoction on Levels and mRNA of IL-6, IL-10 and Protein Expression of HSP70 of Gastric Mucosa in Chronic Atrophic Gastritis Rats with Spleen-Stomach Deficiency / 中国中医药信息杂志

Objective To observe the effects ofXiangsha Liujunzi Decoction (XSLJZ) on levels and mRNA of IL-6, IL-10 and protein expression of HSP70 of gastric mucosa in chronic atrophic gastritis (CAG) rats with spleen-stomach deficiency; To discuss its mechanism.Methods Rats were divided into 2 groups through random number table: normal group and model group. The model of CAG rat with spleen-stomach deficiency type was induced by synthetic methods. After successful modeling, rats were divided into model group, positive control group, XSLJZ high-, medium-, low-dose group. Rats in normal and model group received distilled water 10 mL/(kg?d) for gavage; XSLJZ high-, medium-, low-dose group received XSLJZ 24, 12, 6 g/(kg?d), respectively; positive control group received mycin 0.30 g/(kg?d) for gavage for 120 consecutive days. Generally living conditions, levels and mRNA of IL-6, IL-10 and protein expression of HSP70 in gastric mucosa tissue were detected by protein immunoblotting.Results Compared with the normal group, generally living conditions of rats in the model group were poor; mRNA and the content of IL-6 increased significantly, and mRNA and the content of IL-10 decreased significantly (P<0.05,P<0.01); mRNA and protein expression of HSP70 in gastric tissues was much lower than that of normal group (P<0.05,P<0.01). Compared with model group, generally living conditions of rats in the XSLJZ high-dose groups were improved significantly; mRNA and the content of IL-6 decreased significantly, and mRNA and the content of IL-10 increased significantly (P<0.05); XSLJZ high-, medium-dose groups mRNA and protein expression of HSP70 in gastric tissues increased significantly(P<0.05).Conclusion XSLJZ has protective effects on gastric mucosa of CAG rats with spleen-stomach deficiency.

Effects of Xiangsha Liujunzi decoction on TLR signal pathway in gastric mucosa tissues of rats with Helicobacter pylori-induced chronic atrophic gastritis / 中国中药杂志

To explore the effects and mechanism of Xiangsha Liujunzi decoction on TLR signal pathway in gastric mucosa tissues of rats with Helicobacter pylori-related gastritis, sixty SD rats were randomly divided into control group, model group, high concentration of Xiangsha Liujunzi decoction group, moderate concentration of Xiangsha Liujunzi decoction group, low concentrations of Xiangsha Liujunzi decoction group and SB203580-treated group, with 10 rats in each group. SD rats of Hp-associated chronic atrophic gastritis models were established by intragastric gavage of Helicobacter pylori (HP) suspension. Changes in the gastric mucosa of rats were assessed by histopathology. ELISA was applied to detect the expressions of TNF-α and IL-6 in the serum, and the activity of iNOS in gastric mucosa. The content of NO in the gastric mucosa was tested by nitrate reductive enzymatic. The expressions of TLR2, TLR4, P38MAPK, NF-κB were detected by QPCR and Western-blot. The results indicated that the clinical symptoms of rats and pathological changes of gastric mucosa were improved in Xiangsha Liujunzi decoction group. Compared with normal control group, the protein expressions of TLR2, TLR4, p-P38MAPK and NF-κB in gastric mucosa of model group rats increased (P<0.01) with the levels of TNF-α and IL-6 in the serum, and the activity of iNOS and the content of NO in gastric mucosa increased. Compared with model group, the expressions decreased in Xiangsha Liujunzi decoction group, especially in the high concentration of Xiangsha Liujunzi decoction group(P<0.01), with gradually increased rate of HP eradication and decreased pathological grades of chronic atrophic gastritis. The serum level of TNF-α and IL-6 decreased from (24.313±2.261) μg•L ⁻¹ to (15.195±1.235) μg•L-1(P<0.01) and from (77.416±8.095) μg•L ⁻¹ to (33.150±2.532) μg•L ⁻¹ (P<0.01), and the activity of iNOS and the content of NO in gastric mucosa decreased from (1.530±0.206) U•mg ⁻¹ to (0.802±0.091) U•mg ⁻¹ (P<0.01) and from (0.907±0.032) mmol•g ⁻¹ to (0.335±0.026) mmol•g ⁻¹ (P<0.01) after the treatment of high concentration of Xiangsha Liujunzi decoction. All the effects increased with the increasing dosage of Xiangsha Liujunzi decoction from 0.324 g•mg ⁻¹ to 1.296 g•mg ⁻¹. The protein expressions of NF-κB decreased in the gastric mucosa after treated with P38MAPK specific inhibitor-SB203580. In the rats model, HP infection results in chronic atrophic gastritis through the activation of TLR2, TLR4/MAPK/NF-κB/iNOS/NO signal pathway. Xiangsha Liujunzi decoction can eradicate H. pylori and alleviate chronic atrophic gastric mucosal inflammation. The treatment is effective and safe to cure HP-induced chronic atrophic gastritis.

Clinical efficacy of additive LiuJunZi decoction in treatment of chronic obstructive pneumonia / 中国生化药物杂志

Objective To investigate clinical efficacy of additive LiuJunZi decoction in treatment of patients with chronic obstructive pneumonia ( COPD) of both lung and spleen deficiency in stable phase.Methods A total of 58 patients with deficiency of both lung and spleen type stable COPD patients were randomly divided into treatment group and control group by a random number table, 29 cases in each group, patients in two groups were treated with salmeterol and fluticasone propionate powder for inhalation.On this basis, the treatment group were given additive Liujunzi Decoction, orally, with 3 months treatment.The clinical signs and symptoms of patients, pulmonary function, index of sputumand acute attack proportion in a year were observed and compared.Results After different treatment, total efficiency was 93.1% in treatment group, which was obviously higher than 65.5%in control group (P<0.05).After treatment, the values of FEV1 and FEV1/FVC improved compared with before treatment, and the above indexes of treatment group significantly improved compared with control group (P<0.05).The HDACs activety, levels of TNF-αand IL-8 mproved compared with before treatment, and the above indexes of treatment group significantly improved compared with control group (P<0.05).The acute attack was two cases in treatment group(6.9%), seven cases in control group(24.1%), but the difference had no statistically significant.Conclusion The additive Liujunzi decoction in the treatment of both lung and spleen deficiency type chronic obstructive pulmonary disease ( COPD) in stable phase has exact clinical curative effect, can significantly improve clinical symptoms and laboratory indexes, control the progression of the disease, and improve life quality of the patients.

Chronic Gastritis (Liver Stagnation and Spleen Deficiency Syndrome) 45 Cases Treated by Chaishao Liujunzi Decoction / 浙江中医药大学学报

[Objective] To explore the curative effect of Chaishao Liujunzi Decoction for Chronic Gastritis(Liver Stagnation and Spleen Deficiency Syndrome).[Method] 90 cases were allocated to 2 groups(each group 45 cases) randomly.The treatment group was treated by the Decoction,the control group by Motilium and NexiumTablets.2 weeks constituted the course of treatment.The curative effect,improvement of symptoms and signs,manifestation of endoscopy before and after treatment were observed.[Result] The curative effect in treatment group was significantly different as compared with control group after treatment(P

Effect of Chai Shao Liujunzi Decoction Combined with Entecavir for the Treatment of Chronic Hepatitis B:An Observation of 30 Cases / 广州中医药大学学报

0.05).Normalization rate of ALT was higher in the treatment group than that in the control group (P0.05).The rate of HBeAg turning negative in the treatment group after treatment for one year was higher than that in the control group(P

Content Determination of Polysaccharide in Different Compatibility of Fengshao Liujunzi Decoction / 中国药房

OBJECTIVE: To determine polysaccharide in different compatibility of Fengshao liujunzi decoction.METHODS: Polysaccharide was extracted with water extracting-alcohol precipitation.Polysaccharide was prepared by anthrone-sulfuric acid method and determined by UV spectrophotometry.RESULTS: The optimal determination conditions for polysaccharide in Fengshao liujunzi decoction were as follows: 4 mL 0.2% anthrone-sulfuic acid solution was added into sample,heated in water bath for 15 min then cooled for 10 min.The linear range of D-glucosum anhydricum were 0.019 92～0.079 68 mg(r=0.999 6).The average recovery was 97.33%(RSD=1.52%,n=6).CONCLUSION: Polysaccharide is mainly from Liujunzi decoction.The method is rapid,accurate and reproducible for content determination of polysaccharide in different compatibility of Fengshao liujunzi decoction.

Therapeutic Effect of Spleen-strengthening,Lung-clearing and Phlegm-resolving Therapy for Chronic Obstrustive Pulmonary Disease Complicated with Systemic Inflammatory Response Syndrome and Its Influence on Nuclear Factor ?B Expression / 广州中医药大学学报

Objective To investigate the therapeutic effect of spleen-strengthening,lung-clearing and phlegm-resolving herbs for the treatment of acute attack of chronic obstrustive pulmonary disease(COPD) complicated with systemic inflammatory response syndrome(SIRS),and to observe their effect on nuclear factor ?B(NF-?B)expression level.Methods Sixty qualified patients differentiated as deficiency of spleen and lung,phlegm-heat accumulating the lung were equally randomized into two groups:the control group received routine western medical treatment including oxygen inhalation,antibiotics and bronchodilator,and the treatment group received Chen Xia Liujunzi Decoction and Weijing Decoction,which have the actions of strengthening spleen,clearing lung,and resolving phlegm,one dose per day,for 7 days.The changes of clinical symptoms and signs,blood gas parameters and NF-?B expression level were observed.Results After treatment,clinical symptoms and signs such as cough,expectoration,dyspnea,shortness of breath,and wheeze were relieved in both groups(P

Effects of Xiangsha Liujunzi Decoction on Experimental Bile-Regurgitational Gastritis in Rats / 中药新药与临床药理

The effects of Xiangsha Liujunzi Decoction (XLD) on expermental bile-regurgitational gastritis and gastric evacuation in rats were observed. XLD in dose of 30g/kg (p.o.) markedly pre- vented the acute gastric ucosa damate induced by gastric feeding of alkaline intestinal contents. For chronic regurgitational gastritis rat model induced by pyloric dilation with regurgitation of duodenal juice after 2 and 4 weeks , XLD in dose of 30g/kg (p. o.) lowered the incidence of gastritis, and reduce intragastric pH and bile acid. XLD in dose of 15g/kg and 30g/kg (p.o.) markedly inhibited the gastric evacuation. The results suggested that XLD is available for the treament of bile-regurgi- tational gastritis.